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Partial Rescue of Glomerular Laminin 5 Mutations by Wild-Type Endothelia Produce Hybrid Glomeruli

Dale R. Abrahamson^*, Patricia L. St. John^*, Kathryn Isom^*, Barry Robert and Jeffrey H. Miner

^* Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, Kansas; Comparative Biology Core, Pennington Biomedical Research Center, Baton Rouge, Louisiana; and Renal Division, Department of Internal Medicine, Washington University, St. Louis, Missouri

Correspondence: Dr. Dale R. Abrahamson, Department of Anatomy and Cell Biology, University of Kansas Medical Center, MS 3038, 3901 Rainbow Boulevard, Kansas City, KS 66160. Phone: 913-588-7000; Fax: 913-588-2710; E-mail: dabrahamson{at}kumc.edu

Received for publication February 16, 2007. Accepted for publication April 9, 2007.

Both endothelial cells and podocytes are sources for laminin 1 at the inception of glomerulogenesis and then for laminin 5 during glomerular maturation. Why glomerular basement membranes (GBM) undergo laminin transitions is unknown, but this may dictate glomerular morphogenesis. In mice that genetically lack laminin 5, laminin 521 is not assembled, vascularized glomeruli fail to form, and animals die at midgestation with neural tube closure and placental deficits. It was previously shown that renal cortices of newborn mice contain endothelial progenitors (angioblasts) and that when embryonic day 12 kidneys are transplanted into newborn kidney, hybrid glomeruli (host-derived endothelium and donor-derived podocytes) result. Reasoning that host endothelium may correct the glomerular phenotype that is seen in laminin 5 mutants, 5 null embryonic day 12 metanephroi were grafted into wild-type newborn kidney. Hybrid glomeruli were identified in grafts by expression of a host-specific LacZ lineage marker. Labeling of glomerular hybrid GBM with chain-specific antibodies showed a markedly stratified distribution of laminins: 5 was found only on the inner endothelial half of GBM, whereas 1 located to outer layers beneath mutant podocytes. For measurement of the contribution of host endothelium to hybrid GBM, immunofluorescent signals for laminin 5 were quantified: Hybrid GBM contained approximately 50% the normal 5 complement as wild-type GBM. Electron microscopy of glomerular hybrids showed vascularization, but podocyte foot processes were absent. It was concluded that (1) endothelial and podocyte-derived laminins remain tethered to their cellular origin, (2) developing endothelial cells contribute large amounts of GBM laminins, and (3) podocyte foot process differentiation may require direct exposure to laminin 5.

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Copyright © 2008 by the American Society of Nephrology. Online ISSN: 1533-3450 Print ISSN: 1046-6673