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Origin and Fate of Pendrin-Positive Intercalated Cells in Developing Mouse Kidney

Hyun-Kuk Song^*, Wan-Young Kim^*, Hyun-Wook Lee^*, Eun-Young Park^*, Ki-Hwan Han, Soren Nielsen, Kirsten M. Madsen and Jin Kim^*

^* Department of Anatomy and Cell Death Disease Research Center, The Catholic University of Korea, and Department of Anatomy, Ewha Womans University, Seoul, Korea; Water and Salt Research Center, University of Aarhus, Aarhus, Denmark; and Department of Medicine, University of Florida, Gainesville, Florida

Correspondence: Dr. Jin Kim, Department of Anatomy and Medical Research Center for Cell Death Disease Research Center, College of Medicine, The Catholic University of Korea, Seoul, Korea, 505, Banpo-Dong, Seocho-Ku, Seoul 137-701, Korea. Phone: +82-2-590-1153; Fax: +82-2-590-2992; E-mail: jinkim{at}catholic.ac.kr

Received for publication October 2, 2006. Accepted for publication May 31, 2007.

Pendrin is an apical anion exchanger found in type B and nonA-nonB intercalated cells that is involved in bicarbonate secretion. The purpose of this study was to establish the origin and fate of pendrin-positive intercalated cells in the mouse kidney. Using immunohistochemistry, we found that pendrin-positive cells first appeared in the connecting tubule at embryonic day 14 (E14) and subsequently in the medullary collecting duct at E18. Most of the pendrin-positive cells in the connecting tubule were nonA–nonB intercalated cells, wheras those in the medullary collecting duct were type B intercalated cells. In the cortical collecting duct, pendrin-positive cells appeared in the inner part at day 4 after birth and in the outer part at day 7. Pendrin-positive cells gradually disappeared by apoptosis from the inner part of the medullary collecting duct two weeks after birth. Using 5-bromo-2'deoxy-uridine (BrdU) to follow cell proliferation, we determined that selective proliferation of pendrin-positive intercalated cells does not occur; instead, these cells may arise from undifferentiated precursor cells from separate foci, one in the connecting tubule and one in the collecting duct.

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J. Am. Soc. Nephrol. 2007 18: A13. [Full Text] [PDF]

Copyright © 2008 by the American Society of Nephrology. Online ISSN: 1533-3450 Print ISSN: 1046-6673