Journal of the American Society of Nephrology
2007 JASN IMPACT FACTOR 7.111 HOME   AUTHOR INFO   EDITORIAL BOARD   SUBSCRIBE   FEEDBACK   ALERTS   HELP 
    advanced
CURRENT ISSUE ARCHIVES JASN Express ONLINE SUBMISSION


This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ohse, T.
Right arrow Articles by Nangaku, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ohse, T.
Right arrow Articles by Nangaku, M.
J Am Soc Nephrol 15:919-927, 2004
© 2004 American Society of Nephrology

BASIC SCIENCE

Modulation of Interferon-Induced Genes by Lipoxin Analogue in Anti–Glomerular Basement Membrane Nephritis

Takamoto Ohse*, Tatsuru Ota*, Niamh Kieran{dagger}, Catherine Godson{dagger}, Koei Yamada*, Tetsuhiro Tanaka*, Toshiro Fujita* and Masaomi Nangaku*

*Division of Nephrology and Endocrinology, University of Tokyo School of Medicine, Tokyo, Japan; {dagger}Department of Medicine and Therapeutics, Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Mater Misericordiae Hospital, and Dublin Molecular Medicine Center, Dublin, Ireland.

Corrrespondence to Dr. Masaomi Nangaku, Division of Nephrology and Endocrinology, University of Tokyo School of Medicine, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan. Phone: 81-3-5800-8648; Fax: 81-3-5800-8806; E-mail: mnangaku-tky{at}umin.ac.jp

ABSTRACT. Immune complex deposition is associated with the accumulation of neutrophils, which play an important role in the various immune-mediated diseases. A novel anti-inflammatory agent, the lipoxin A (LXA) analogue (15-epi-16-(FPhO)-LXA-Me)), a stable synthetic analogue of aspirin-triggered 15-epi-lipoxin A4 (ATLa), was used in experimental anti–glomerular basement membrane (GBM) antibody nephritis in mice. ATLa was administered before the induction of the disease, and 2 h later, the animals were killed. ATLa reduced the infiltrating neutrophils and nitrotyrosine staining in glomeruli. Subsequent changes of gene expression in the early phase were evaluated, and 5674 genes were present under the basal conditions in kidneys from normal mice; 54 upregulated genes and 25 downregulated genes were detected in anti-GBM nephritis. Eighteen of these upregulated genes were those induced by IFN-{gamma}. Real-time quantitative PCR analysis confirmed the results of the microarrays. To investigate a role of IFN-{gamma} in neutrophil infiltration, anti-GBM nephritis was induced in IFN-{gamma} knockout mice. The number of infiltrating neutrophils in these mice did not differ from those in wild-type mice. Also examined were CD11b expression on neutrophils from mice treated with ATLa by flow cytometry, but suppression of this adhesion molecule was not observed. Neutrophil infiltration was successfully inhibited by ATLa in the early phase of murine anti-GBM nephritis. Microarray analysis detected the change of mRNA expression in anti-GBM nephritis and demonstrated amelioration of various genes by ATLa, which may provide a clue to the development of novel therapeutic approaches in immune renal injury.




This article has been cited by other articles:


Home page
 Am. J. Pathol.Home page
S.-H. Wu, P.-Y. Liao, P.-L. Yin, Y.-M. Zhang, and L. Dong
Elevated Expressions of 15-Lipoxygenase and Lipoxin A4 in Children with Acute Poststreptococcal Glomerulonephritis
Am. J. Pathol., January 1, 2009; 174(1): 115 - 122.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
K. Doi, K. Okamoto, K. Negishi, Y. Suzuki, A. Nakao, T. Fujita, A. Toda, T. Yokomizo, Y. Kita, Y. Kihara, et al.
Attenuation of Folic Acid-Induced Renal Inflammatory Injury in Platelet-Activating Factor Receptor-Deficient Mice
Am. J. Pathol., May 1, 2006; 168(5): 1413 - 1424.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
R. Inagi, Y. Yamamoto, M. Nangaku, N. Usuda, H. Okamato, K. Kurokawa, C. van Ypersele de Strihou, H. Yamamoto, and T. Miyata
A Severe Diabetic Nephropathy Model With Early Development of Nodule-Like Lesions Induced by Megsin Overexpression in RAGE/iNOS Transgenic Mice
Diabetes, February 1, 2006; 55(2): 356 - 366.
[Abstract] [Full Text] [PDF]


HOME CURRENT ISSUE ARCHIVES JASN Express ONLINE SUBMISSION AUTHOR INFO
EDITORIAL BOARD SUBSCRIBE FEEDBACK ALERTS HELP

Copyright © 2008 by the American Society of Nephrology. Online ISSN: 1533-3450 Print ISSN: 1046-6673