Journal of the American Society of Nephrology
2007 JASN IMPACT FACTOR 7.111 HOME   AUTHOR INFO   EDITORIAL BOARD   SUBSCRIBE   FEEDBACK   ALERTS   HELP 
    advanced
CURRENT ISSUE ARCHIVES JASN Express ONLINE SUBMISSION


This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Krüger, T.
Right arrow Articles by Kurts, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Krüger, T.
Right arrow Articles by Kurts, C.
J Am Soc Nephrol 15:613-621, 2004
© 2004 American Society of Nephrology

BASIC SCIENCE

Identification and Functional Characterization of Dendritic Cells in the Healthy Murine Kidney and in Experimental Glomerulonephritis

Thilo Krüger*, Dirk Benke{dagger}, Frank Eitner*, Andreas Lang*, Monika Wirtz*, Emma E. Hamilton-Williams{dagger}, Daniel Engel{dagger}, Bernd Giese{ddagger}, Gerhard Müller-Newen{ddagger}, Jürgen Floege* and Christian Kurts{dagger}

Departments of *Nephrology and Clinical Immunology and {ddagger}Biochemistry, University Clinic of Aachen, Aachen, Germany; and {dagger}Institute of Molecular Medicine and Experimental Immunology, University Clinic of Bonn, Bonn, Germany.

Correspondence to Dr. Christian Kurts, Institute of Molecular Medicine and Experimental Immunology, Friedrich-Wilhelms-Universität, 53105 Bonn, Germany. Phone: 49-228-287-1031; Fax: 49-228-287-1052; E-mail: ckurts{at}web.de

ABSTRACT. The kidney tubulointerstitium contains numerous bone marrow-derived antigen-presenting cells, which are often referred to as resident tissue macrophages, although several previous studies had demonstrated characteristics of dendritic cells (DC). In this study, we describe a subset of tubulointerstitial cells expressing the DC marker CD11c. A protocol was established to isolate these cells for in vitro analysis. Renal CD11c+ cells resembled splenic DC, but not peritoneal macrophages, in morphology, lysosomal content, phagocytic activity, microbicidal effector functions, expression of T cell costimulatory molecules, and ability to activate T cells. Nevertheless, many CD11c+ renal cells expressed low or intermediate levels of F4/80 and CD11b, indicating that both markers are not absolutely specific for macrophages in the kidney. Subpopulations of renal DC could be distinguished based on their expression of MHC class II and costimulatory molecules and may represent different maturation stages. In nephrotoxic glomerulonephritis, increased numbers of CD11c+ cells showing DC functionality were found in the tubulointerstitium. Focal accumulation was seen within tubulointerstitial mononuclear infiltrates and adjacent to, but not within, inflamed glomeruli. These results are the first to identify and characterize renal CD11c+ cells as DC and to demonstrate marked changes in experimental glomerulonephritis.




This article has been cited by other articles:


Home page
 J. Immunol.Home page
D. R. Engel, J. Maurer, A. P. Tittel, C. Weisheit, T. Cavlar, B. Schumak, A. Limmer, N. van Rooijen, C. Trautwein, F. Tacke, et al.
CCR2 Mediates Homeostatic and Inflammatory Release of Gr1high Monocytes from the Bone Marrow, but Is Dispensable for Bladder Infiltration in Bacterial Urinary Tract Infection
J. Immunol., October 15, 2008; 181(8): 5579 - 5586.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
N. Eter, D. R. Engel, L. Meyer, H.-M. Helb, F. Roth, J. Maurer, F. G. Holz, and C. Kurts
In Vivo Visualization of Dendritic Cells, Macrophages, and Microglial Cells Responding to Laser-Induced Damage in the Fundus of the Eye
Invest. Ophthalmol. Vis. Sci., August 1, 2008; 49(8): 3649 - 3658.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
V. W. S. Lee, Y. M. Wang, Y. P. Wang, D. Zheng, T. Polhill, Q. Cao, H. Wu, I. E. Alexander, S. I. Alexander, and D. C. H. Harris
Regulatory immune cells in kidney disease
Am J Physiol Renal Physiol, August 1, 2008; 295(2): F335 - F342.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
R. Veelken, E.-M. Vogel, K. Hilgers, K. Amann, A. Hartner, G. Sass, W. Neuhuber, and G. Tiegs
Autonomic Renal Denervation Ameliorates Experimental Glomerulonephritis
J. Am. Soc. Nephrol., July 1, 2008; 19(7): 1371 - 1378.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
K. L. Edgtton, J. Y. Kausman, M. Li, K. O'Sullivan, C. Lo, P. Hutchinson, H. Yagita, S. R. Holdsworth, and A. R. Kitching
Intrarenal Antigens Activate CD4+ Cells via Co-stimulatory Signals from Dendritic Cells
J. Am. Soc. Nephrol., March 1, 2008; 19(3): 515 - 526.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
J. Scholz, V. Lukacs-Kornek, D. R. Engel, S. Specht, E. Kiss, F. Eitner, J. Floege, H.-J. Groene, and C. Kurts
Renal Dendritic Cells Stimulate IL-10 Production and Attenuate Nephrotoxic Nephritis
J. Am. Soc. Nephrol., March 1, 2008; 19(3): 527 - 537.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
V. Lukacs-Kornek, S. Burgdorf, L. Diehl, S. Specht, M. Kornek, and C. Kurts
The Kidney-Renal Lymph Node-System Contributes to Cross-Tolerance against Innocuous Circulating Antigen
J. Immunol., January 15, 2008; 180(2): 706 - 715.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. Menke, J. A. Lucas, G. C. Zeller, M. E. Keir, X. R. Huang, N. Tsuboi, T. N. Mayadas, H. Y. Lan, A. H. Sharpe, and V. R. Kelley
Programmed Death 1 Ligand (PD-L) 1 and PD-L2 Limit Autoimmune Kidney Disease: Distinct Roles
J. Immunol., December 1, 2007; 179(11): 7466 - 7477.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
R. John and P. J. Nelson
Dendritic Cells in the Kidney
J. Am. Soc. Nephrol., October 1, 2007; 18(10): 2628 - 2635.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
U. Panzer, O. M. Steinmetz, H.-J. Paust, C. Meyer-Schwesinger, A. Peters, J.-E. Turner, G. Zahner, F. Heymann, C. Kurts, H. Hopfer, et al.
Chemokine Receptor CXCR3 Mediates T Cell Recruitment and Tissue Injury in Nephrotoxic Nephritis in Mice
J. Am. Soc. Nephrol., July 1, 2007; 18(7): 2071 - 2084.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
D. Engel, U. Dobrindt, A. Tittel, P. Peters, J. Maurer, I. Gutgemann, B. Kaissling, W. Kuziel, S. Jung, and C. Kurts
Tumor Necrosis Factor Alpha- and Inducible Nitric Oxide Synthase-Producing Dendritic Cells Are Rapidly Recruited to the Bladder in Urinary Tract Infection but Are Dispensable for Bacterial Clearance
Infect. Immun., November 1, 2006; 74(11): 6100 - 6107.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
R. D. Pawar, P. S. Patole, D. Zecher, S. Segerer, M. Kretzler, D. Schlondorff, and H.-J. Anders
Toll-Like Receptor-7 Modulates Immune Complex Glomerulonephritis
J. Am. Soc. Nephrol., January 1, 2006; 17(1): 141 - 149.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
A. Lang, D. Benke, F. Eitner, D. Engel, S. Ehrlich, M. Breloer, E. Hamilton-Williams, S. Specht, A. Hoerauf, J. Floege, et al.
Heat Shock Protein 60 Is Released in Immune-Mediated Glomerulonephritis and Aggravates Disease: In Vivo Evidence for an Immunologic Danger Signal
J. Am. Soc. Nephrol., February 1, 2005; 16(2): 383 - 391.
[Abstract] [Full Text] [PDF]


HOME CURRENT ISSUE ARCHIVES JASN Express ONLINE SUBMISSION AUTHOR INFO
EDITORIAL BOARD SUBSCRIBE FEEDBACK ALERTS HELP

Copyright © 2008 by the American Society of Nephrology. Online ISSN: 1533-3450 Print ISSN: 1046-6673