2007 JASN IMPACT FACTOR 7.111	HOME   AUTHOR INFO   EDITORIAL BOARD   SUBSCRIBE   FEEDBACK   ALERTS   HELP
advanced	CURRENT ISSUE	ARCHIVES	JASN Express	ONLINE SUBMISSION

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by O’Riordan, E. Articles by Goligorsky, M. S. Search for Related Content PubMed PubMed Citation Articles by O’Riordan, E. Articles by Goligorsky, M. S. Related Collections Related Article

Bioinformatic Analysis of the Urine Proteome of Acute Allograft Rejection

Edmond O’Riordan^*, Tatyana N. Orlova^*, Jianfeng Mei J, Khalid Butt, Praveen M. Chander, Shafiq Rahman^*, Muong Mya^*, Rena Hu^*, Jahangir Momin^*, Elizabeth W. Eng^*, Dierk J. Hampel^||, Bertram Hartman^¶, Matthias Kretzler^¶, Veronica Delaney^* and Michael S. Goligorsky^*

*Renal Research Institute and Division of Nephrology, Department of Medicine, New York Medical College, Department of Pathology, and Department of Surgery, Division of Transplantation, New York Medical College, Valhalla, New York; Ciphergen Biosystems, Inc., Fremont, California; ^||Division of Nephrology and Medical Intensive Care, Charite, Campus Virchow-Clinic-University Medicine, Berlin, Germany; and ^¶University of Munich School of Medicine, Division of Nephrology, Munich, Germany

Correspondence to Dr. Edmond O’Riordan, Renal Research Institute, Room C06, Basic Science Building, New York Medical College, 95 Grasslands Road, Valhalla, NY 10595. Phone: 914-594-4733; Fax: 914-594-4732; E-mail edmond_oriordan{at}nymc.edu

The urinary proteome in health and disease attracts increasing attention because of the potential diagnostic and pathophysiologic biomarker information carried by specific excreted proteins or their constellations. This cross-sectional study aimed to analyze the urinary proteome in patients with biopsy-proven acute rejection (n = 23) compared with transplant recipients with stable graft function (n = 22) and healthy volunteers (n = 20) and to correlate this with clinical, morphologic, and laboratory data. Urine samples were preadsorbed on four different protein chip surfaces, and the protein composition was analyzed using a surface-enhanced laser desorption/ionization time-of-flight mass spectrometer platform. The data were analyzed using two independent approaches to sample classification. Patients who experienced acute rejection could be distinguished from stable patients with a sensitivity of 90.5 to 91.3% and a specificity of 77.2 to 83.3%, depending on the classifier used. Protein masses that were important in constructing the classification algorithms included those of mass 2003.0, 2802.6, 4756.3, 5872.4, 6990.6, 19,018.8, and 25,665.7 Da. Normal urine was distinguished from transplant urine using a protein marker of mass 78,531.2 Da with both a sensitivity and a specificity of 100%. In conclusion, (1) urine proteome in transplant recipients with stable graft function was significantly different from healthy control subjects, and (2) acute rejections were characterized by a constellation of excreted proteins. Analysis of the urinary proteome may expedite the noninvasive prediction of acute graft rejection, thus importantly assisting in establishing the diagnosis.

Related Article

This Month’s Highlights
J. Am. Soc. Nephrol. 2004 15: 2955-2958. [Full Text] [PDF]

This article has been cited by other articles:

				S. Decramer, A. G. de Peredo, B. Breuil, H. Mischak, B. Monsarrat, J.-L. Bascands, and J. P. Schanstra Urine in Clinical Proteomics Mol. Cell. Proteomics, October 1, 2008; 7(10): 1850 - 1862. [Abstract] [Full Text] [PDF]

				H. Dihazi, G. A. Muller, S. Lindner, M. Meyer, A. R. Asif, M. Oellerich, and F. Strutz Characterization of Diabetic Nephropathy by Urinary Proteomic Analysis: Identification of a Processed Ubiquitin Form as a Differentially Excreted Protein in Diabetic Nephropathy Patients Clin. Chem., September 1, 2007; 53(9): 1636 - 1645. [Abstract] [Full Text] [PDF]

				J. Barratt MB ChB (Ho and P. Topham MB ChB MD Urine proteomics: the present and future of measuring urinary protein components in disease Can. Med. Assoc. J., August 14, 2007; 177(4): 361 - 368. [Abstract] [Full Text] [PDF]

				M. S. Goligorsky, F. Addabbo, and E. O'Riordan Diagnostic Potential of Urine Proteome: A Broken Mirror of Renal Diseases J. Am. Soc. Nephrol., August 1, 2007; 18(8): 2233 - 2239. [Abstract] [Full Text] [PDF]

				G. A. Muller, C. A. Muller, and H. Dihazi Clinical proteomics--on the long way from bench to bedside? Nephrol. Dial. Transplant., May 1, 2007; 22(5): 1297 - 1300. [Full Text] [PDF]

				K. Mosley, F. W. K. Tam, R. J. Edwards, J. Crozier, C. D. Pusey, and L. Lightstone Urinary proteomic profiles distinguish between active and inactive lupus nephritis Rheumatology, December 1, 2006; 45(12): 1497 - 1504. [Abstract] [Full Text] [PDF]

				T. Jahnukainen, D. Malehorn, M. Sun, J. Lyons-Weiler, W. Bigbee, G. Gupta, R. Shapiro, P. S. Randhawa, R. Pelikan, M. Hauskrecht, et al. Proteomic Analysis of Urine in Kidney Transplant Patients with BK Virus Nephropathy J. Am. Soc. Nephrol., November 1, 2006; 17(11): 3248 - 3256. [Abstract] [Full Text] [PDF]

				T. Pisitkun, R. Johnstone, and M. A. Knepper Discovery of Urinary Biomarkers Mol. Cell. Proteomics, October 1, 2006; 5(10): 1760 - 1771. [Abstract] [Full Text] [PDF]

				R. B. Mannon and A. D. Kirk Beyond Histology: Novel Tools to Diagnose Allograft Dysfunction Clin. J. Am. Soc. Nephrol., May 1, 2006; 1(3): 358 - 366. [Abstract] [Full Text] [PDF]

Copyright © 2008 by the American Society of Nephrology. Online ISSN: 1533-3450 Print ISSN: 1046-6673