2007 JASN IMPACT FACTOR 7.111	HOME   AUTHOR INFO   EDITORIAL BOARD   SUBSCRIBE   FEEDBACK   ALERTS   HELP
advanced	CURRENT ISSUE	ARCHIVES	JASN Express	ONLINE SUBMISSION

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wan Kim, S. Articles by Lee, J. Search for Related Content PubMed PubMed Citation Articles by Wan Kim, S. Articles by Lee, J.

Indomethacin Enhances Shuttling of Aquaporin-2 Despite Decreased Abundance in Rat Kidney

Soo Wan Kim^*, Joon Wan Kim, Ki Chul Choi^*, Seong Kwon Ma^*, YoonWha Oh, Ju-Young Jung, Jin Kim and JongUn Lee

Departments of *Internal Medicine and Physiology, Chonnam National University Medical School, and Department of Anatomy, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.

Correspondence to Dr. JongUn Lee, Department of Physiology, Chonnam National University Medical School, 5 Hak-dong, Gwangju 501-746, Korea. Phone: +82 62 220 4262; Fax: +82 62 232 1242; E-mail: julee{at}chonnam.ac.kr

The effect of nonsteroidal antiinflammatory drugs on the regulation of aquaporin-2 (AQP2) water channels in the kidney was determined. Male Sprague-Dawley rats were injected with indomethacin (5 mg/kg twice a day intraperitoneally) for 2 d. The control group was injected with vehicle. The expression of AQP2 proteins was determined in the kidney by immunoblotting and immunohistochemistry. The expression of G_s and type VI adenylyl cyclase was determined by immunoblotting. The activity of adenylyl cyclase complexes was determined by stimulated accumulation of cAMP. Immunoblotting revealed that indomethacin markedly decreased the expression of AQP2. Accordingly, however, the ratio of AQP2 expression in the membrane fraction versus that in the cytoplasmic fraction was increased. The urinary excretion of AQP2 proteins also increased. Immunohistochemistry demonstrated almost exclusive apical labeling of AQP2 with scanty cytoplasmic localization along the collecting duct. The expression of G_s and adenylyl cyclase VI proteins was decreased. The generation of cAMP provoked by arginine vasopressin, sodium fluoride, or forskolin was blunted. These results suggest that indomethacin increases the shuttling of AQP2 while it decreases its abundance in the collecting duct.

This article has been cited by other articles:

				C. R. J. Kennedy, H. Xiong, S. Rahal, J. Vanderluit, R. S. Slack, Y. Zhang, Y. Guan, M. D. Breyer, and R. L. Hebert Urine concentrating defect in prostaglandin EP1-deficient mice Am J Physiol Renal Physiol, February 1, 2007; 292(2): F868 - F875. [Abstract] [Full Text] [PDF]

				J. Xu, R. T. Worrell, H. C. Li, S. L. Barone, S. Petrovic, H. Amlal, and M. Soleimani Chloride/Bicarbonate Exchanger SLC26A7 Is Localized in Endosomes in Medullary Collecting Duct Cells and Is Targeted to the Basolateral Membrane in Hypertonicity and Potassium Depletion J. Am. Soc. Nephrol., April 1, 2006; 17(4): 956 - 967. [Abstract] [Full Text] [PDF]

Copyright © 2008 by the American Society of Nephrology. Online ISSN: 1533-3450 Print ISSN: 1046-6673