2007 JASN IMPACT FACTOR 7.111	HOME   AUTHOR INFO   EDITORIAL BOARD   SUBSCRIBE   FEEDBACK   ALERTS   HELP
advanced	CURRENT ISSUE	ARCHIVES	JASN Express	ONLINE SUBMISSION

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by COBO, A. Articles by WERUAGA, A. Search for Related Content PubMed PubMed Citation Articles by COBO, A. Articles by WERUAGA, A.

Growth Plate Cartilage Formation and Resorption Are Differentially Depressed in Growth Retarded Uremic Rats

ANGELES COBO^*, JOSÉ LÓPEZ, EDUARDO CARBAJO, FERNANDO SANTOS^*, JESÚS ALVAREZ, MARTA FERNÁNDEZ and ANA WERUAGA^*

^* Department of Pediatrics, School of Medicine, University of Oviedo, Oviedo, Asturias, Spains.
Department of Cell Biology, School of Medicine, University of Oviedo, Oviedo, Asturias, Spain.
Department of Anatomy, School of Medicine, University of Oviedo, Oviedo, Asturias, Spain.

Correspondence to Dr. José M. López, Department of Morfología y Biología Celular, Facultad de Medicina, C/Julián Clavería 6, 33006 Oviedo, Asturias, Spain. Phone: 34 98 5103064; Fax: 34 98 5103618; E-mail: JMLOPEZ{at}sci.cpd.uniovi.es

Abstract. To characterize the modifications of growth plate in individuals with growth impairment secondary to chronic renal failure, young rats were made uremic by subtotal nephrectomy (NX) and, after 14 d, their tibial growth plates were studied and compared with those of sham-operated rats fed ad libitum (SAL) or pair-fed with NX (SPF). NX rats were growth retarded and severely uremic. Growth plate height (mean ± SD) was much greater (P < 0.05) in NX (868.4 ± 85.4 µm) than SAL (570.1 ± 93.5 µm) and SPF (551.9 ± 99.7 µm) rats as a result of a higher (P < 0.05) hypertrophic zone (661.0 ± 89.7 versus 362.8 ± 71.6 and 353.0 ± 93.9 µm, respectively). The increased size of the growth plate was associated with a greater number of chondrocytes and modifications in their structure, particularly in the hypertrophic zone adjacent to bone. In this zone, chondrocytes of NX animals were significantly (P < 0.05) smaller (12080.4 ± 1158.3 µm³) and shorter (34.1 ± 2.5 µm) than those of SAL (16302.8 ± 1483.4 µm³ and 37.8 ± 2.0 µm) and SPF (14465.8 ± 1521.0 µm³ and 36.3 ± 1.8 µm). The interface between the growth plate cartilage and the metaphyseal bone appeared markedly irregular in NX rats. Kinetics of chondrocytes was also modified (P < 0.05) in the NX rats, which had lower cell turnover per column per day (5.4 ± 0.9), longer duration of hypertrophic phase (89.0 ± 15.2 h), and reduced cellular advance velocity (7.4 ± 2.2 µm/h) compared with SAL (8.0 ± 1.6, 32.1 ± 6.7 h, and 11.3 ± 2.7 µm/h) and SPF (7.2 ± 1.1, 34.8 ± 5.1 h, and 10.1 ± 2.5 µ/h). Cell proliferation was no different among the three groups. Because the growth plates of SPF and SAL rats were substantially not different, modifications observed in the NX rats cannot be attributed to the nutritional deficit associated with renal failure. These findings indicate that chronic renal failure depresses both the activity of the growth plate cartilage by altering chondrocyte hypertrophy and the replacement of cartilage by bone at the metaphyseal end. The two processes are differentially depressed since cartilage resorption is more severely lowered than cartilage enlargement and this leads to an accumulation of cartilage at the hypertrophic zone.

This article has been cited by other articles:

				P. M. Mandalunis and A. M. Ubios Experimental Renal Failure and Iron Overload: A Histomorphometric Study in Rat Tibia Toxicol Pathol, April 1, 2005; 33(3): 398 - 403. [Abstract] [Full Text] [PDF]

				E. CARBAJO, J. M. LÓPEZ, F. SANTOS, F. A. ORDÓÑEZ, P. NIÑO, and J. RODRÍGUEZ Histologic and Dynamic Changes Induced by Chronic Metabolic Acidosis in the Rat Growth Plate J. Am. Soc. Nephrol., June 1, 2001; 12(6): 1228 - 1234. [Abstract] [Full Text]

Copyright © 2008 by the American Society of Nephrology. Online ISSN: 1533-3450 Print ISSN: 1046-6673