2007 JASN IMPACT FACTOR 7.111	HOME   AUTHOR INFO   EDITORIAL BOARD   SUBSCRIBE   FEEDBACK   ALERTS   HELP
advanced	CURRENT ISSUE	ARCHIVES	JASN Express	ONLINE SUBMISSION

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by SHARMA, M. Articles by SAVIN, V. J. Search for Related Content PubMed PubMed Citation Articles by SHARMA, M. Articles by SAVIN, V. J.

"The FSGS Factor"

Enrichment and in Vivo Effectof Activity from Focal Segmental Glomerulosclerosis Plasma

Division of Nephrology, Department of Medicine, Medical College of Wisconsin, Milwaukee, Wisconsin.

Correspondence to Dr. Mukut Sharma, Medical College of Wisconsin, Room 466-C, CVRC/MEB (Nephrology), 8701, Watertown Plank Road, Milwaukee, WI 53226. Phone: 414-456-8805; Fax: 414-456-6515; E-mail: msharma{at}mcw.edu

Abstract. A circulating causative factor has been postulated in focal segmental glomerulosclerosis (FSGS). It has been shown that serum or plasma from some FSGS increases glomerular albumin permeability (P_alb) in vitro. P_alb greater than 0.5 (i.e., FS activity) is associated with recurrence after transplantation. Specimens from 15 FSGS patients were studied to document the presence of a permeability factor, to isolate this factor, to characterize its biochemical properties, and to show its effect in vivo. Total lipids were extracted by chloroform/methanol (2:1); FS activity was absent from total lipid extract. Chylomicrons and lipoproteins were removed from the plasma with dextran sulfate, followed by sequential precipitation of proteins at 50 and 70% ammonium sulfate saturation. FS activity was retained in the 70% ammonium sulfate supernatant and exhibited a 100-fold purification. FS activity was lost after heating at 100°C for 10 min or after protease digestion. Under nondenaturing conditions, electrophoresis of the FSGS 70% supernatant showed a prominent low molecular weight band that was not evident in the 70% supernatant from normal plasma. Dialysis and centrifugation-based membrane ultrafiltration of the FSGS factor indicated a molecular size between 30 and 50 kD. Injection of the 70% FSGS supernatant into rats caused a threefold increase in urine protein in collections from 6 to 24 h after injection. No increase in proteinuria occurred in rats injected with 70% supernatant from normal individuals. It is concluded that the FSGS factor is a low molecular weight protein with the potential to increase P_alb in vitro and to cause proteinuria in vivo.

This article has been cited by other articles:

				B. Haraldsson, J. Nystrom, and W. M. Deen Properties of the Glomerular Barrier and Mechanisms of Proteinuria Physiol Rev, April 1, 2008; 88(2): 451 - 487. [Abstract] [Full Text] [PDF]

				I. Londono and M. Bendayan Glomerular handling of native albumin in the presence of circulating modified albumins by the normal rat kidney Am J Physiol Renal Physiol, December 1, 2005; 289(6): F1201 - F1209. [Abstract] [Full Text] [PDF]

				M. Carraro, C. Zennaro, M. Artero, G. Candiano, G. M. Ghiggeri, L. Musante, C. Sirch, M. Bruschi, and L. Faccini The effect of proteinase inhibitors on glomerular albumin permeability induced in vitro by serum from patients with idiopathic focal segmental glomerulosclerosis Nephrol. Dial. Transplant., August 1, 2004; 19(8): 1969 - 1975. [Abstract] [Full Text] [PDF]

				R. G. Ruf, A. Lichtenberger, S. M. Karle, J. P. Haas, F. E. Anacleto, M. Schultheiss, I. Zalewski, A. Imm, E.-M. Ruf, B. Mucha, et al. Patients with Mutations in NPHS2 (Podocin) Do Not Respond to Standard Steroid Treatment of Nephrotic Syndrome J. Am. Soc. Nephrol., March 1, 2004; 15(3): 722 - 732. [Abstract] [Full Text] [PDF]

				M. Sharma, R. Sharma, E. T. McCarthy, and V. J. Savin The Focal Segmental Glomerulosclerosis Permeability Factor: Biochemical Characteristics and Biological Effects Experimental Biology and Medicine, January 1, 2004; 229(1): 85 - 98. [Abstract] [Full Text] [PDF]

				G. Caridi, R. Bertelli, M. Di Duca, M. Dagnino, F. Emma, A. Onetti Muda, F. Scolari, N. Miglietti, G. Mazzucco, L. Murer, et al. Broadening the Spectrum of Diseases Related to Podocin Mutations J. Am. Soc. Nephrol., May 1, 2003; 14(5): 1278 - 1286. [Abstract] [Full Text] [PDF]

				D. Cattran, T. Neogi, R. Sharma, E. T. McCarthy, and V. J. Savin Serial Estimates of Serum Permeability Activity and Clinical Correlates in Patients with Native Kidney Focal Segmental Glomerulosclerosis J. Am. Soc. Nephrol., February 1, 2003; 14(2): 448 - 453. [Abstract] [Full Text] [PDF]

				M. R. Pollak Inherited Podocytopathies: FSGS and Nephrotic Syndrome from a Genetic Viewpoint J. Am. Soc. Nephrol., December 1, 2002; 13(12): 3016 - 3023. [Full Text] [PDF]

				M. Imgrund, R. Rohrbach, M.-O. Oksuz, and W. Grotz Unusual course of nephrotic syndrome associated with atypical pneumonia Nephrol. Dial. Transplant., August 1, 2002; 17(8): 1530 - 1532. [Full Text] [PDF]

				R. Sharma, M. Sharma, P. K. Datta, and V. J. Savin Induction of Metallothionein-I Protects Glomeruli from Superoxide-Mediated Increase in Albumin Permeability Experimental Biology and Medicine, January 1, 2002; 227(1): 26 - 31. [Abstract] [Full Text] [PDF]

				G. M. Ghiggeri, M. Artero, M. Carraro, and F. Perfumo Permeability plasma factors in nephrotic syndrome: more than one factor, more than one inhibitor Nephrol. Dial. Transplant., May 1, 2001; 16(5): 882 - 885. [Full Text] [PDF]

				M. J. Kemper, G. Wolf, and D. E. Muller-Wiefel Transmission of Glomerular Permeability Factor from a Mother to Her Child N. Engl. J. Med., February 1, 2001; 344(5): 386 - 387. [Full Text] [PDF]

				G. CANDIANO, L. MUSANTE, M. CARRARO, L. FACCINI, L. CAMPANACCI, C. ZENNARO, M. ARTERO, F. GINEVRI, F. PERFUMO, R. GUSMANO, et al. Apolipoproteins Prevent Glomerular Albumin Permeability Induced In Vitro by Serum from Patients with Focal Segmental Glomerulosclerosis J. Am. Soc. Nephrol., January 1, 2001; 12(1): 143 - 150. [Abstract] [Full Text]

Copyright © 2008 by the American Society of Nephrology. Online ISSN: 1533-3450 Print ISSN: 1046-6673